產(chǎn)品原理
本實驗采用雙抗體夾心ELISA法??笻IV-1 Gag p24單抗包被于酶標板上,同時加入標本、標準品和生物素化的抗HIV-1 Gag p24抗體,標本、標準品中的HIV-1 Gag p24會與加入于孔中的生物素化的抗HIV-1 Gag p24抗體及包被于酶標板上的單抗結(jié)合,形成免疫復(fù)合物,游離的成分被洗去;加入辣根過氧化物酶標記的親合素,親合素與生物素特異性結(jié)合,游離的成分被洗去。加入顯色底物(顯色劑),若反應(yīng)孔中有HIV-1 Gag p24,辣根過氧化物酶會使無色的顯色劑變藍,加終止液變黃。在450nm 處測OD值,HIV-1 Gag p24 濃度與OD450值之間呈正相關(guān),可通過繪制標準曲線求出標本中HIV-1 Gag p24度。
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