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ResiQuant? ready-to-use HEK 293 DNA Residue Detection Kit Product Introduction（CRH00-1081）

ResiQuant? ready-to-use HEK 293 DNA Residue Detection Kit Product Introduction（CRH00-1081）

ResiQuant? ready-to-use HEK 293 DNA Residue Detection Kit Product Introduction

Catalog No.
Size
CRH00-1081
 50T
CRH00-1082
 100T


Overview performance Related application image Data and files literature Q&A COA

This kit uses the Taqman probe quantitative PCR method to quickly detect residual human embryonic kidney cell 293 (HEK 293) host cell DNA in intermediates, semi-finished products and finished products of various biological products and pharmaceuticals. The kit introduces an uracil-N-glycosylase (UNG) system, which can effectively remove residual contamination of PCR products and great reduce false positives caused by contamination of amplified products. The reagent components contain internal standard control (IC), we can monitor whether the reaction process is proceeding normally and eliminate sample interference; ROX is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays a role in optical path correction.
This product is suitable for biology drug solutions, finished products, semi-finished products, intermediate products or process monitoring samples produced with human embryonic kidney cell 293 stain as host. To obtain a calibration curve for quantitative sample DNA, the kit contains DNA calibrators with 5 concentration gradients (300 pg/μL to 30 fg/μL) for preparation of calibration curve.
For the extraction of residual DNA from HEK293 host cells, it is recommended to use our company’s universal residual host DNA sample pretreatment kit. Before first use, it is recommended to complete a product suitability study: confirm whether there are interferences or inhibitors in the sample or sample matrix, and select appropriate sample detection dilution conditions.

Product Features
1.Quantitative Range: 30 fg/μL to 300 pg/μL;
2.Precision: repeatability coefficient of variation within the limit of quantitation ≤15%，intermediate precision variation coefficient ≤30%;
3.Exclusive: Specific detection of residual DNA in 293 host cells without interference from E.coli, Vero, CHO and other foreign cells;
 
Product Advantages
High Accuracy
The DNA calibrator has a complete traceability chain
Strong Anti-interference
UNG Anti-pollution System and Digestion Procedure：Improve the kit’s tolerance to aerosol contamination of PCR products；
ROX: The kit contains ROX dye and fluorescence reference calibration optical pathlength
Internal Control (IC)：Improve detection control of sample interference and reaction preparation;
Supply chain security
Large batch output, short delivery time, stable supply;
Ready-to-use calibrator
No need for cumbersome dilution operations, simplifying the experimental process; avoid artificial differences in the standard dilution operation, and the results are consistent;