產(chǎn)品概述
IL-1包含兩種不同基因的編碼的蛋白，IL-1α和IL-1β，但是這兩種亞型具有25%的氨基酸序列同源性，并且它們可識別相同的細胞受體。已知的分泌IL-1的細胞有成骨細胞，單核細胞，巨噬細胞，角質(zhì)形成細胞，Kupffer細胞，肝細胞，胸腺和唾液腺上皮，雪旺氏細胞，成纖維細胞和神經(jīng)膠質(zhì)細胞（少突神經(jīng)膠質(zhì)，星形膠質(zhì)細胞和小膠質(zhì)細胞）。IL-1α和IL-1β都是由31KDa的前體蛋白合成。在不同的物種中，成熟的IL-1α的氨基酸序列有60%到70%的保守性，已發(fā)現(xiàn)人的IL-1可以作用于鼠的細胞系。
產(chǎn)品原理
本實驗采用雙抗體夾心ELISA法?？勾笫驣L-1α單抗包被于酶標板上，同時加入標本、標準品和生物素化的抗大鼠IL-1α抗體，標本、標準品中的IL-1α會與加入于孔中的生物素化的抗大鼠IL-1α抗體及包被于酶標板上的單抗結合，形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有IL-1α，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值， IL-1α濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中IL-1α濃度。
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