產品概述
IL-3是一種多效性的細胞因子，可刺激細胞的增殖分化并刺激多功能造血干細胞和譜系定向祖細胞的存活。表達IL-3的細胞有幾種造血和非造血細胞包括激活后的人的T細胞，自然殺傷性細胞，肥大細胞，上皮細胞，基質細胞，鼠角質細胞，神經(jīng)元和星形膠質細胞。人的IL-3的核苷酸序列是由133個氨基酸組成的多肽序列和19個氨基酸組成的信號序列。在氨基酸水平上，小鼠和人的氨基酸序列只有29%的同源性，雖然同源性比較低，但是在他們在功能中具體種屬特異性。IL-3不僅對造血干細胞有影響，還可以刺激人的內皮細胞和平滑肌細胞的增殖和運動。IL-3被證明可以作為神經(jīng)營養(yǎng)因子促進神經(jīng)元的存活和神經(jīng)突的生長。
產品原理
本實驗采用雙抗體夾心ELISA法?？谷薎L-3單抗包被于酶標板上，標本和標準品中的IL-3會與單抗結合形成免疫復合物，游離的成分被洗去；加入生物素化的抗人IL-3抗體，生物素化抗人IL-3抗體與酶標板上結合的標準品或樣本中的IL-3結合而形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有IL-3，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，IL-3濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中IL-3濃度。
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